Assembling & Annotating Microbial Genomes

Running assembly and annotation in KBase

KBase enables de novo assembly of prokaryotic NGS reads from various sequencing platforms. Assemblies can then be annotated with RAST or Prokka, enabling you to explore structural and functional features of a Genome or use it in other analyses.

Steps for assembly and annotation include:

1. Upload reads to assemble.

2. Add your Reads data object to the Narrative.

3. Search the App Catalog and insert an Assembly App

   • Use one of these tools to create an Assembly object.

4. Search the App Catalog and insert an Annotation App

   • Use one of these tools to annotate the Assembly object.

5. Examine assembly job statistics and genome annotation.

Narrative Tutorials

• Genome Analysis 1: Drafting Isolate Genomes – demonstrates how to draft isolate genomes from quality assessment to assembly and annotation.

Video Tutorial

Introduction to Genome Analysis